Ozone therapy improves early stages of osseointegration in ovariectomized rats.

OBJECTIVE: the aim of this study was to analyze the influence of ozone therapy (OZN) on peri-implant bone repair in critical bones by installing osseointegrated implants in the tibia of ovariectomized rats. METHODOLOGY: ovariectomy was performed on 30 Wistar rats, aged six months (Rattus novergicus), and, after 90 days, osseointegrated implants were installed in each tibial metaphysis. The study groups were divided into the animals that received intraperitoneal ozone at a concentration of 700 mcg/kg - OZ Group (n=15) - and a control group that received an intraperitoneal saline solution and, for this reason, was named the SAL group (n=15). The applications for both groups occurred during the immediate post-operative period on the 2nd, 4th, 6th, 8th, 10th, and 12th day post-surgery. At various stages (14, 42, and 60 days), the animals were euthanized, and tests were performed on their tibiae. These tests include histomorphometric and immunohistochemical analyses, computerized microtomography, sampling in light-cured resin for calcified sections, and confocal microscopy. The obtained data were then analyzed using One-way ANOVA and the Shapiro-Wilk, Kruskal-Wallis, and student t-tests (P<0.05). RESULTS: our findings indicate that the OZ group (3.26±0.20 mm) showed better cellular organization and bone neoformation at 14 days (SAL group, 0.90±1.42 mm) (P=0.001). Immunohistochemistry revealed that osteocalcin labeling was moderate in the OZ group and mild in the SAL group at 14 and 42 days post-surgery. The data from the analysis of calcified tissues (microtomography, histometric, and bone dynamism analysis) at 60 days showed no statistically significant differences between the groups (P=0.32). CONCLUSION: it was concluded that ozone therapy anticipated the initial phases of the peri-implant bone repair process.

Evaluation of Porcine Collagen Membranes Used with Guided Bone Regeneration for Critical Defects: A Histological, Histomorphometric, Immunohistochemical, and Inflammatory Profile Analysis.

OBJECTIVE: The objective of this study was to compare the effectiveness of two porcine collagen membranes of different origin used for guided bone regeneration procedures. MATERIALS AND METHODS: Resorbable collagen membrane from porcine dermis (Bio-Gide, Geistlich Pharma AG, Wolhusen, Switzerland) and resorbable collagen membrane from porcine pericardium (Jason, Institut Straumann AG, Peter Merian-Weg, Switzerland) were evaluated; histological, histometric, immunohistochemical, and inflammatory profile analyses were performed. The study was carried out on critical defects created in the calvaria of 72 rats (Rattus norvegicus albinus, Wistar variety) divided into three groups: coagulum group (Co), porcine pericardium group (JS), and porcine collagen group (BG). The defects were filled with clot, over which the membranes were placed. The animals were euthanized 7, 15, 30, and 60 days after surgery. STATISTICAL ANALYSIS: The Shapiro-Wilk test was used to assess data distribution. Analysis of variance (ANOVA) and the Bonferroni multiple comparison test were used to compare the differences across the mean values of the variables. Nonparametric tests, Mann-Whitney and Wilcoxon W, were used for the quantitative analysis of the inflammatory profile. A significance level of 5% (p < 0.05) was adopted with a confidence interval of 95%. SPSS software version 2.0 was used. RESULTS: A total of 1,008 analyses were performed on 288 histological slides. It was noted that both types of collagen membranes used in this study were effective for the guided bone regeneration procedure, with a greater proportion and thickness of bone formation among recipients of the BG (735 points, p = 0.021). This membrane also had greater permeability (62.25). The animals in the JS group, which received the porcine pericardial membrane, showed early and accelerated bone formation from early bone tissue, milder osteopontin and osteocalcin levels, and greater inflammatory reaction (86.4). CONCLUSION: The collagen membrane from porcine dermis demonstrated a more orderly and physiological repair process, while the porcine pericardial membrane presented a more accelerated repair process that did not remain constant over time.

Ozone therapy improves early stages of osseointegration in ovariectomized rats

Abstract Objective the aim of this study was to analyze the influence of ozone therapy (OZN) on peri-implant bone repair in critical bones by installing osseointegrated implants in the tibia of ovariectomized rats. Methodology ovariectomy was performed on 30 Wistar rats, aged six months (Rattus novergicus), and, after 90 days, osseointegrated implants were installed in each tibial metaphysis. The study groups were divided into the animals that received intraperitoneal ozone at a concentration of 700 mcg/kg — OZ Group (n=15) — and a control group that received an intraperitoneal saline solution and, for this reason, was named the SAL group (n=15). The applications for both groups occurred during the immediate post-operative period on the 2nd, 4th, 6th, 8th, 10th, and 12th day post-surgery. At various stages (14, 42, and 60 days), the animals were euthanized, and tests were performed on their tibiae. These tests include histomorphometric and immunohistochemical analyses, computerized microtomography, sampling in light-cured resin for calcified sections, and confocal microscopy. The obtained data were then analyzed using One-way ANOVA and the Shapiro-Wilk, Kruskal-Wallis, and student t-tests (P<0.05). Results our findings indicate that the OZ group (3.26±0.20 mm) showed better cellular organization and bone neoformation at 14 days (SAL group, 0.90±1.42 mm) (P=0.001). Immunohistochemistry revealed that osteocalcin labeling was moderate in the OZ group and mild in the SAL group at 14 and 42 days post-surgery. The data from the analysis of calcified tissues (microtomography, histometric, and bone dynamism analysis) at 60 days showed no statistically significant differences between the groups (P=0.32). Conclusion it was concluded that ozone therapy anticipated the initial phases of the peri-implant bone repair process.

What are the Consequences of Incorrect Handling in the Treatment of Blunt-Cut Lip Injuries?

This study aimed report two cases of blunt-cut lip injury which occurred in postoperatory dehiscence. Patients were referred to the emergence attendance and the clinical examination revealed extensive laceration on the upper and lower lips of both patients, highly contaminated with asphalt residue. The affected regions were properly cleaned with saline and antiseptic solution, and, under local anesthesia, suturing was performed in layers in the muscle and oral mucosa region with 4-0 resorbable thread and in the skin region with 6-0 non-absorbable thread. Patients presented different levels of dehiscence on post-operatory evaluation. Thus, it was prescribed antibiotic and healing ointments for decontamination and improvement of the healing process. Third days post-operatory one of the patients presented a great healing of external lips and vermilion lip. However, the other patient presented aesthetic sequelae in the vermilion region of the lip, with a line of fibrosis and misalignment in the region, but not function sequelae. Thus, it was concluded that blunt injuries on the lip must strictly follow the established treatment protocols and must begin aiming for favorable results in the first intervention. Moreover, the postoperative care of the patient and the surgeon are as important as the procedure itself.

Calvaria Critical Size Defects Regeneration Using Collagen Membranes to Assess the Osteopromotive Principle: An Animal Study.

Guided bone regeneration (GBR) is a common practice in implantology, and it is necessary to use membranes in this process. The present study aimed to evaluate the osteopromotive principle of two porcine collagen membranes in critical-size defects at rats calvaria. Ninety-six Albinus Wistar rats were divided into BG (positive control), JS, CS, and CG (negative control) groups and were sacrificed at 7, 15, 30, and 60 days postoperatively. The samples were assessed by histological, histometric, immunohistochemical, and microtomographic analyses. More intense inflammatory profile was seen in the JS and CS groups (p < 0.05). At 60 days, the JS group showed a satisfactory osteopromotive behavior compared to BG (p = 0.193), while CS did not demonstrate the capacity to promote bone formation. At the immunohistochemical analysis, the CS showed mild labeling for osteocalcin (OC) and osteopontin (OP), the JS demonstrated mild to moderate for OC and OP and the BG demonstrated moderate to intense for OC and OP. The tridimensional analysis found the lowest average for the total volume of newly formed bone in the CS (84,901 mm2), compared to the BG (319,834 mm2) (p < 0.05). We conclude that the different thicknesses and treatment techniques of each membrane may interfere with its biological behavior.

Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen.

The membranes are an important biomaterial that contribute to osteopromotion. This study aimed to evaluate the osteopromotive potential of collagen membranes associated with Hydroxyapatite (HA) in critical size calvaria rat's defects. Ninety-six Albinus Wistar rats were divided into four groups: (CG) negative control: clot only (CG); positive control: porcine collagen membrane (BG); fish collagen membrane associated with HA (CP); bovine collagen membrane associated with HA (CB), analyzed at 7, 15, 30, and 60 postoperative days. At 30 days, membrane integrity was observed in the CB and fragments in the CP and BG groups were dispersed in the center of the defect. At 60 days, BG demonstrated better results with no statistical difference for the CP group (p = 0.199) and a statistically significant difference for the CB group (p = 0.013). The inflammatory profiles of the BG and CP groups were similar. Immunohistochemistry demonstrated at 60 days moderate osteopontin staining for the BG and CP groups, light staining for the CB, and intense osteocalcin staining for the BG, while the CB and CP groups demonstrated moderate staining. Microtomography revealed the highest mean bone volume (14.247 mm3) in the BG, followed by the CB (11.850 mm3), and CP (9.560 mm3) group. The collagen membranes associated with HA demonstrated an osteopromotive potential.

Single-Dose Ibuprofen-Arginine as a Preventive for Pain, Edema, and Trismus After Impacted Lower Third Molar Surgery: A Randomized Split-Mouth Clinical Trial.

OBJECTIVE: We examined if the association of ibuprofen with arginine has a better anti-inflammatory effect on pain, edema, and trismus after surgery of the impacted mandibular third molar than ibuprofen alone. MATERIALS AND METHODS: The study included 21 patients, 18 to 30 years of age, each with an impacted, and bilateral and symmetric third molar (total n = 21) that required transalveolar extraction. Patients were randomly assigned numbers from 1 to 21. Group A received ibuprofen-arginine as preoperative medication, while Group B received only ibuprofen. Both groups received the same postoperative medications: amoxicillin + acetaminophen. All patients were evaluated for pain at 6, 12, and 24 hours. They were evaluated for edema and trismus before surgery; immediately after surgery; and at 24, 48, and 72 hours postoperatively. Postoperative pain scores used the visual analog scale (BS-11). For facial edema and trismus, linear measurements used the method modified by Gabka and Matsumura. STATISTICAL ANALYSIS: For the evaluation of data between Group A and Group B, we used the statistical software SPSS version 22. The Shapiro-Wilk, analysis of variance, the Bonferroni comparisons, and the Wilcoxon test were used. All tests were based on a significance level of 0.05. RESULTS: The study results reveal that the facial edema scores of Group A and Group B presented statistically significant differences (p < 0.05), while for postoperative trismus, there was no statistically significant difference (p > 0.05) between the scores of Group A and Group B. CONCLUSION: As a conclusion, we can state that the use of ibuprofen-arginine allows for significantly better control of pain and edema, and shows a tendency toward better recovery from trismus, although without statistical significance. Based on this, we can assert that arginine improves the anti-inflammatory power of ibuprofen, thus generating better tissue healing after surgery of the impacted third molar.

A triple-blind randomized clinical trial of different associations between dexamethasone and non-steroids anti-inflammatories for preemptive action in third molar extractions.

The aim of this study is to evaluate the preemptive analgesic effects of dexamethasone (DEX) alone or combined with non-steroidal anti-inflammatory drugs (NSAIDs) in third molar surgeries. The subjects were divided into five groups (n = 20 teeth/group); subjects received only 8 mg of dexamethasone 1 h before the surgical procedure (DEX group), or in combination with etodolac (DEX + ETO), ketorolac (DEX + KET), ibuprofen (DEX + IBU), loxoprofen (DEX + LOX). Paracetamol 750 mg was provided as the number of rescue analgesics (NRA). Salivary PGE2 expression was measured preoperatively and at 48 h. Edema and Maximum mouth opening (MMO) were measured postoperatively at 48 h and 7 days. A visual analog scale (VAS) was performed postoperatively at 6, 12, 24, 48, 72 h, and 7 days. Salivary expression of PGE2 showed a decrease only for the DEX group. Edema and MMO and NRA consumption showed no significant differences among the groups (P > 0.05). The VAS showed a significantly lower pain perception at 6 h after the surgery for the DEX + ETO and DEX + KET groups (P < 0.05). The combination of DEX and NSAIDS should be considered for preemptive acute postsurgical pain management in third molar surgery. In some drug associations such as dexamethasone 8 mg + NSAIDS (ETO and KET) in the pre-operative time, only a few rescue analgesics are necessary.

Evaluation of Osteoconduction of a Synthetic Hydroxyapatite/ß-Tricalcium Phosphate Block Fixed in Rabbit Mandibles.

(1) Background: This study aimed to evaluate the incorporation of hydroxyapatite/ß-tricalcium phosphate blocks grafted in rabbit mandibles. (2) Methods: Topographic characterization of biomaterial was performed through scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM-EDX). Ten rabbits randomly received autogenous bone graft harvested from the tibia (Autogenous Group-AG) or synthetic biomaterial manufactured in ß-tricalcium phosphate (Biomaterial Group-BG) at their right and left mandibular angles. Euthanasia was performed at 30 and 60 postoperative days; (3) Results: SEM-EDX showed a surface with the formation of crystals clusters. Histological analyses in BG at 30 days showed a slower process of incorporation than AG. At 60 days, BG showed remnants of biomaterial enveloped by bone tissue in the anabolic modeling phase. Histometric analysis showed that mean values of newly formed bone-like tissue in the AG (6.56%/9.70%) were statistically higher compared to BG (3.14%/6.43%) in both periods, respectively. Immunohistochemical analysis demonstrated early bone formation and maturation in the AG with more intense osteopontin and osteocalcin staining. (4) Conclusions: The biomaterial proved to be a possible bone substitute, being incorporated into the receiving bed; however, it showed delayed bone incorporation compared to autogenous bone.

Osteopromotion Capacity of Bovine Cortical Membranes in Critical Defects of Rat Calvaria: Histological and Immunohistochemical Analysis.

Membranes that aid the guided bone regeneration (GBR) process have been the subject of studies of compatible biomaterials that contribute to this repair process. The present study compared different membranes used in critical-size defects of rat calvaria by assessing GBR as well as histological, histomorphometric, and immunohistochemical reactions. Forty-eight male albino Wistar rats were randomly allocated into four groups (n = 12 each), namely, C: membrane-free control group (only blood clot, negative control group); BG: porcine collagen membrane group (Bio-Gide®, positive control group); GD: bovine cortical membrane group (first experimental group); and GDF: thicker bovine cortical membrane group (second experimental group). Rats were euthanized at 30 and 60 days postoperatively. Quantitative data from the histometric analysis were submitted to two-way ANOVA and Tukey's posttest when p < 0.05. Histomorphometric results of the thicker bovine cortical membrane at 30 and 60 days were promising, showing improved new bone formation values (p < 0.05), and the CD group presented similar results in both analysis periods, being surpassed only by the GDF group (p < 0.05). The immunohistochemical results were associated with the histomorphometric data. A less-thick membrane also assisted in GBR. All membranes promoted GBR, especially the positive control and experimental groups.

Bone tissue formation around two titanium implant surfaces placed in bone defects filled with bone substitute material or blood clot: A pilot study.

OBJECTIVES: The objective of this study was to evaluate the peri-implant bone tissue formation around titanium implants with different surface treatments, placed in bone defects filled or not with bone substitute material (BSM). MATERIALS AND METHODS: Ten animals were divided into two groups according to implant surface treatment. In each tibia, a bone defect was created followed by the placement of one implant. On the left tibia, the defect was filled with blood clot (BC), and on the right tibia, the defect was filled with biphasic hydroxyapatite/ß-tricalcium-phosphate (HA/TCP) generating four subgroups: BC-N: blood clot and porous surface; BC-A: blood clot and porous-hydrophilic surface; HA/TCP-N: BSM and porous surface; HA/TCP-A: BSM and porous-hydrophilic surface. The animals were submitted to euthanasia 60 days after implant installation. After light-curing resin inclusion, the blocks containing the implant and the bone tissue were stained and evaluated by means of histomorphometry to assess the percentages of bone implant contact (% BIC). Data was normally distributed and the group differences were examined using the parametric tests of Two-Way ANOVA. RESULTS: The BC-A group presented the higher mean value of BIC (46.43%). The HA/TCP-A group presented the higher mean value of BIC. The porous-hydrophilic surfaces presented better results of BIC when compared to the porous surface in both conditions of defect filling. No statistically significant differences were found among all groups (95% confidence interval and P < .05). CONCLUSION: According to histomorphometric analysis, after 60-days in a rabbit model, hydrophilic and hydrophobic surfaces have the same behavior in the presence or absence of HA/TCP.

Implantes dentários curtos na implantodontia moderna: revisão sistematizada / Short implants dental in modern implantology: systematized review / Implantes dentales curtos en la implantodontia moderna: revisión sistematizada

Introdução: Os implantes curtos têm ganhado espaço na implantodontia moderna devido se tratar de uma técnica que apresenta baixa morbidade, sem necessidade de cirurgias de enxertos prévias e menor custo para o paciente, quando comparada a outras técnicas. Objetivo: Dessa forma, foi realizada uma revisão da literatura atual sobre as vantagens e indicações desta técnica comparando-a as técnicas convencionais. Materiais e métodos: A pesquisa bibliográfica foi realizada usando MEDLINE via PubMed, utilizando termos e outros termos livres dos Cabeçalhos de Assunto Médicos (MeSH) para pesquisar. Abrangendo artigos publicados em inglês, dos ultimos 18 anos. Resultados: Foram selecionados 56 referências, das quais 26 foram consideradas inadequadas. Resultando no total de 30 artigos considerados adequados aos critérios de seleção. Os artigos selecionados apresentaram em alguns pontos divergência que ainda necessitam de respostas baseadas em evidencias. Conclusão: Conclui-se com esta revisão que os implantes curtos são uma boa alternativa quando não se tem altura óssea suficiente para instalação de implantes convencionais, e que apresenta vantagens e indicações restritas no universo da implantodontia com resultados similares quando comparados aos implantes de tamanhos convencionais. Porém, apresentam resultados a curto prazo o que sugere a realização de estudos com longo tempo de acompanhamento que busquem responder sobre a qualidade da técnica nos diversos desafios encontrados na implantodontia(AU)

Introduction: The short implants have an open space in modern implantology due to a technique that presents low morbidity, without previous grafting surgeries and lower cost for the patient, when compared to other techniques. Objective: Thus, a review of current literature on the advantages and indications of technique was carried out comparing conventional techniques. Materials and methods: Bibliographic research was conducted using MEDLINE via PubMed, using terms and other free Medical Subject Headings (MeSH) terms to search for. Covering articles published in English, from the last 18 years. Results: We selected 56 references, of which 26 were considered inadequate. Resulting in a total of 30 articles considered adequate to the selection criteria. The selected articles presented in some points divergence that still need evidence based answers. Conclusion: It was concluded with this review that short implants are a good alternative when there is not enough height for the installation of conventional implants, and that they have advantages and restricted indications no universe of the implantology with similar results when compared to the implants of conventional sizes. However, results of a short term and that suggests a course of studies with a time of accompaniment that seek answers on a quality of the technique in the diverse challenges found in the implantology(AU)

Introducción: Los implantes cortos han ganado espacio en la implantodoncia moderna debido a que se trata de una técnica que presenta baja morbilidad, sin necesidad de cirugías de injertos previos y menor costo para el paciente, en comparación con otras técnicas. Objetivo: De esta forma, se realizó una revisión de la literatura actual sobre las ventajas e indicaciones de esta técnica comparándola con las técnicas convencionales. Materiales y métodos: La investigación bibliográfica fue realizada usando MEDLINE vía PubMed, utilizando términos y otros términos libres de los Cabeceros de Asunto Médicos (MeSH) para investigar. Abriendo artículos publicados en inglés, de los últimos 18 años. Resultados: Se seleccionaron 56 referencias, de las cuales 26 fueron consideradas inadecuadas. Resultando en el total de 30 artículos considerados adecuados a los criterios de selección. Los artículos seleccionados presentaron en algunos puntos divergencia que aún necesitan respuestas basadas en evidencias. Conclusión: Se concluye con esta revisión que los implantes cortos son una buena alternativa cuando no se tiene suficiente altura ósea para instalación de implantes convencionales y que presenta ventajas e indicaciones restringidas en el universo de la implantodoncia con resultados similares en comparación con los implantes de tamaños convencionales. Sin embargo, presentan resultados a corto plazo lo que sugiere la realización de estudios con largo tiempo de acompañamiento que busquen responder sobre la calidad de la técnica en los diversos desafíos encontrados en la implantodoncia(AU)

Taxa de sobrevivência de implantes e biomateriais no levantamento do seio maxilar com pneumatização significativa: um estudo retrospectivo com 79 pacientes, durante seis meses a dez anos / Survival rate of immediate dental implants and biomaterials after large sinus lifting procedures: a retrospective study on 79 patients with a 6-month to 10-year follow-up

Objetivo: avaliar retrospectivamente a taxa de sucesso de implantes e biomateriais após o levantamento do seio maxilar. Material e métodos: foram utilizados prontuários de pacientes tratados entre 1998 e 2014, incluídos os que possuíam rebordo maxilar com altura menor do que 5 mm. Todos os procedimentos de enxertia foram realizados com instalação de implante de forma mediata e acesso ao seio maxilar pela janela óssea lateral, colocação do biomaterial, membrana e sutura. Resultados: foram analisados 79 pacientes, tendo sido utilizados dois biomateriais. O tempo de reavaliação variou de seis meses a dez anos. Nos dados de altura óssea radiográfica antes e depois do procedimento (70 implantes, 36 pacientes), houve um aumento médio de altura na região do seio maxilar com o Bio-Oss de 17,23 mm e com o Orthogen de 13,12 mm (p < 0,05), sendo a média geral de 15,17 mm. Na relação sobrevida do implante e enxerto utilizado (92 implantes, 43 pacientes), os valores foram de 96,8% (autógeno) e 98,3% (autógeno + biomaterial). Conclusão: os resultados permitiram concluir que tanto os enxertos autógenos como os biomateriais são alternativas viáveis para os seios maxilares com grande pneumatização.

Objective: to retrospectively evaluate the success rate of implants and biomaterials after maxillary sinus lift. Material and methods: records of patients treated between 1998 and 2014 were used, including those with maxillary ridge height less than 5 mm. All grafting procedures were performed with implant placement and access to the maxillary sinus through the lateral bony window, placement of the biomaterial, membrane and suture. Results: 79 patients were analyzed and two biomaterials were used. The follow-up period ranged from six months to ten years. In the radiographic bone height data before and after the procedure (70 implants, 36 patients), there was a mean increase in height in the maxillary sinus region with the Bio-Oss of 17.23 mm and with the Orthogen of 13.12 mm (p < 0.05), the overall mean being 15.17 mm. Regarding implant and graft survival (92 implants, 43 patients), the values were 96.8% (autogenous) and 98.3% (autogenous + biomaterial). Conclusion: both autogenous grafts and biomaterials are viable alternatives for the maxillary sinuses with great pneumatization.

Avaliação in vivo do torque de inserção do novo sistema de implantes Emfils / In vivo insertion torque assessment of the new dental implant system Emfi ls

Objetivo: avaliar o torque de inserção de um novo desenho de implante. Material e métodos: pacientes foram recrutados para instalação de implantes, no período de março a dezembro de 2015. Dados como o diâmetro, o comprimento do implante, a sequência de fresagem, a área de instalação e o torque atingido foram considerados. A avaliação do torque foi realizada com um torquímetro cirúrgico. Resultados: foram avaliados 82 implantes, com diâmetros entre 3,5 mm e 5 mm, e comprimentos entre 7 mm e 16 mm, sendo instalados 46 implantes na mandíbula e 36 implantes na maxila. Os torques (média ± desvio-padrão) alcançados foram de 53 ± 9,9 Ncm para maxila e 57 ± 11,3 Ncm para mandíbula. Conclusão: o sistema de implantes Novo Colosso promoveu torque de inserção adequado, com boa estabilidade primária nos implantes.

Objective: to evaluate the insertion torque of a new implant design. Material and methods: patients were recruited for implant placement from March to December 2015. Data such as diameter, implant length, drilling sequence, installation area, and torque reached were considered. Torque evaluation was performed with a surgical torque wrench. Results: 82 implants were evaluated, with diameters between 3.5 mm and 5.0 mm, and lengths between 7 mm and 16 mm, with 46 implants in the mandible and 36 implants in the maxilla. The torques (mean ± standard deviation) reached 53 ± 9.9 Ncm for maxilla and 57 ± 11.3 Ncm for mandible. Conclusion: the Novo Colosso implant system promotes adequate insertion torque with good primary implant stability.

Avaliação histomorfométrica e de imonoistoquimica de membranas absorvíveis para regeneração óssea guiada instaladas em defeitos ósseos críticos de calvárias de ratos / Histomorphometric and immunohistochemical assessment of absorbable membranes for guided bone regeneration in critical-size defects of rat calvaria

O uso de membranas que auxiliem no processo de regeneração óssea guiada (ROG) é também uma vertente dos estudos de biomateriais compatíveis que auxiliam nesse processo de reparo. Objetivo: Avaliar por meio de estudo microscópico, histomorfométrico e imunoistoquimico a regeneração ! óssea guiada utilizando membranas de origens diferentes em defeitos críticos criados em calvária de ratos. Materiais e métodos: Foram utilizados 48 ratos Albinus Wistar divididos em 4 grupos, sendo 6 animais para cada grupo: grupo controle somente com coágulo; grupo controle positivo (Bio-Gide®), grupo experimental 1 que recebeu a membrana cortical de osso bovino mais delgada (Gen-Derm®) e o grupo experimental 2 que recebeu a membrana de cortical bovina mais espessa (Gen-Derm Flex®). Os períodos experimentais foram de 30e 60 dias. Os resultados histomorfométricos deste trabalho apontaram que a membrana de cQJágeno suíno juntamente com a membrana de cortical bovina de maior espessura apresentaram melhores índices de neoformação óssea. Resultado esse também confirmado pela imunoistoquimica. Contudo a membrana de cortical bovina de menor espessura também auxiliou no processo de ROG. Conclusão: todas as membranas estudadas nesta pesquisa promoveram a ROG, sendo que esse processo foi melhor nos grupos controle positivo e experimental 2(AU)

The use of membranes that aid in the guided bane regeneration (GBR) process is also a part of compatible biomaterials studies that aid in this repair process. Objective: To compare different membranes used in critical-size defect of rat calvaria through assessment of GBR, histomorphometric and immunohistochemical reactions. Materials and Methods: 48 male albinus wistar rats were divided into four groups (n=6): control group without membrane (only blood clot/negative control); porcine collagen membrane group (Bio- Gide®/positive control); group that received bovine cortical membrane (Gen- Derm®/experimental 1 group) and group that received ticker bovine cortical membrane (Gen-Derm Flex®/ experimental 2 group). The animals were euthanized at 30 and 60 days postoperatively. The histomorphometric and immunohistochemical results showed that purcine collagen and thicker bovine cortical membranes presented better newly bone formation values, however the less thick membrane also assisted in the GBR. Conclusion: Ali membranes analysed in this study promoted guided bone regeneration, which this process was better in the positive control and experimental 2 groups(AU)

Comparative Assessment of the Effect of Ibuprofen and Etodolac on Edema, Trismus, and Pain in Lower Third Molar Surgery: A Randomized Clinical Trial.

PURPOSE: To compare the efficacy of ibuprofen (IBU) and etodolac (ETO) for controlling pain, edema, and trismus after extraction of lower third molars. MATERIALS AND METHODS: Twenty adolescents and adults with 2 impacted mandibular-third molars (in similar positions) were selected for the study. Patients were randomly assigned either to the IBU group (600 mg of IBU 3 times a day for 3 days) or to the ETO group (300 mg of ETO 3 times a day for 3 days). Drugs were administered immediately after dental extraction. RESULTS: During the first 2 days after extraction, swelling was more pronounced in the IBU group than in the ETO group (P = .033). Seven days after surgery, there was no difference in the degree of edema between the groups. At the 2- and 7-day evaluation points, mouth opening was significantly more reduced in the IBU group than in the ETO group (P < .05). After the first 6 hours, the ETO group had more effective pain relief (P < .05), but after this time point, both groups reported similar degrees of relief. Compared with the IBU group, the ETO group had a lower need for administration of additional rescue analgesics. CONCLUSIONS: After extraction of impacted lower third molars, we found that swelling, trismus, and pain were more effectively controlled with ETO than with IBU.